Microglia, one of the glial cell types in the central nervous system (CNS), is an important integral component of the neuro-glial cell network [1]. They have been observed in the brain parenchyma from the early stage of development to the mature state. Microglia play an important role in brain immune surveillance. They can present antigens in the molecular context of MHC class II expression to CD-4 positive T cells, and are capable of Fc-mediated phagocytosis, and share many common antigens with hematopoietic and tissue macrophages [2]. Upon activation, they act as brain macrophages to clear tissue debris, damaged cells, or microbes, when programmed cell death occurs during brain development or when the CNS is injured.
Recommended Medium
It is recommended to use Microglia Medium (MM, Cat. No. SC1901) for culturing Mouse Microglia in vitro.
Product Use
Mouse Microglia are for research use only.
They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References:
[1] Lee SC, Liu W, Brosnan CF,Dickson DW. (1992) "Characterization of primary human fetal dissociated central nervous system cultures with an emphasis on microgia."Laboratory Investigation. 67:465-76.[2] Fedoroff S, Zhai R,Novak JP. (1997) "Microglia and astroglia have a common progenitor cell."J Neurosci Res. 50: 477-86.[3] Stoll G,Jander S. (1999) "The role of microglia and macrophages in the pathophysiology of the CNS."Prog Neurobiol. 58:233-47.