Mouse Embryonic Fibroblasts (MEF) are used to support the growth of undifferentiated mouse or human ES and iPS cells. They provide both a substrate for the ES cells to grow on and secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos and are used at their early passages. These cells have been mitomycin treated to prevent further cell division prior to cryopreservation.
Recommended Medium
It is recommended to use DMEM (Cat. No. SC09221) supplemented with 10% fetal bovine serum (FBS, Cat. No. SC0010, SC0025, SC0500) for culturing MEF-mt in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] BRADLEY, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. [2] Nagy et al. (2006) Preparing Mouse Embryo Fibroblasts Cold Spring Harbor Protocols. 2006: pdb.prot 4398.