Meningeal cells surround the brain and actively participate in the development of the central nervous system. They play an important role in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, and laminating the cerebellar cortex [1]. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerebellar cortex and dentate gyrus [1]. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex, in adult rat spinal cord lesions promotes axonal regrowth [2]. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells [3].
Recommended Medium
It is recommended to use Meningeal Cell Medium (MCM, Cat. No. SC1401) for culturing MMC in vitro.
Product Use
MMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
[1] Hartmann D, Sievers J, Pehlemann FW, Berry M. (1992) “Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus.” J Comparative Neurol. 320: 33-61.
[2] Franzen R, Martin D, Daloze A, Moonen G, Schoenen J. (1999) “Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth.” Neuroreport. 10: 1551-6.
[3] Hartmann D, Schulze M, Sievers J. (1998) “Meningeal cells stimulate and direct the migration of cerebellar external granule cells in vitro.” J Neurocytol. 27: 395-409.