Lens epithelial cells(LEpiC) are responsible for homeostasis regulation of the lens, including electrolyte and fluid transport [1]. Under normal development, LEpiC progressively differentiate and mature. LEpiC then migrate from the equatorial region into the interior of the lens to produce transparent crystallins, elongate to form lens fiber cells, and eventually lose their nuclei and other organelles [2]. Studies have shown that LEpiC differentiation and lens polarization are regulated by growth factors present in the ocular fluids [3], such as epidermal growth factor, basic fibroblast growth factor, insulin growth factor, and insulin [4].
Recommended Medium: It is recommended to use Epithelial Cell Medium (EpiCM Cat. no. SC4101) for culturing MLEpiC in vitro.
Product Use: MLEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen, and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Hejtmancik J and Shiels A. (2015) “Overview of the Lens” Prog Mol Biol Transl Sci. 134:119-127.
[2] Wagner LM, Takemoto DJ. (2001) “PKCa and PKCg overexpression causes lentoid body formation in the N/N 1003A rabbit lens epithelial cell line.” Molecular Vision. 7: 138-44.
[3] Lang RA. (1999) “Which factors stimulate lens fiber cell differentiation in vivo?” Invest Opthalmol Vis Sci. 40: 3075-8.
[4] Leenders WP, van Genesen ST, Schoenmakers JG, van Zoelen EJ, Lubsen NH. (1997) “Synergism between temporally distinct growth factors: bFGF, insulin and lens cell differentiation.” Mech Dev. 67: 193-201.