Name: Superoxide Dismutase Assay
SKU: SC8198
Price: 338.00 EUR
Availability: InStock

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Cell based assay

Catalog number: SC8198

Unit size: 100 test/kit

Superoxide dismutase (SOD), as one of the body’s most important defense mechanisms against free-radical damage, catalyzes the dismutation of the superoxide radical (O2-) into hydrogen peroxide (H2O2) and elemental oxygen (O2). In ScienCell’s SODAssay, the superoxide anions, generated from the conversion of xanthine to uric acid and hydrogen peroxide by xanthine oxidase (XOD), reduce WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) to water-soluble formazans, which can be measured by absorbance at 438 nm. SODs lower the rate of the reduction reaction by reducing superoxide anion concentrations. Therefore, the % Inhibition of the reduction reaction can be determined as a measurement of SOD activity.

Article number:SC8198

Unit:pcs

Stock status:In stock

338,00€ / pcs

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Description

Quality Control: Serially diluted SOD solutions with concentrations ranging from 0.625 to 40 units/ml are measured with the ScienCell™SOD Assay after different time of reaction, and the resulting standard curves are shown in Figure 1. % Inhibition of reduction reaction can be calculated based on the corresponding Δ A438nm/minfor each SOD concentration. Positive linear relationship between % Inhibition & logarithm of SOD concentration to the base 10 (Log [SOD concentration]) can be observed within the range of 0.625 to 10 units/ml (Figure 2).