Total Glutathione Assay
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Total Glutathione Assay

Metabolic Assay

Reduced glutathione (GSH) is a tripeptide (g-glutamylcysteinylglycine) and plays a key role in preventing oxidative damage in living organisms. GSH is commonly the most abundant low molecular mass thiol in living cells. Intracellular GSH status appears to be a sensitive indicator of the redox state of a cell. High levels of GSH have been proven to correlate with good health and longevity, where as low levels of GSH are believed to contribute to many medical conditions, such as heart attacks and neurodegenerative diseases. This colorimetric assay is based on the reaction between 5’, 5’-Dithiobis 2-nitrobenzoic acid (DTNB) and GSH to form TNB, which exhibits maximum absorbance at 412 nm. The intensity of the absorbance is proportional to the GSH level in the sample. Since glutathione reductase is used in this assay, the level of GSH in the sample reflects the total glutathione (GSSG+GSH) level.

Catalog number: SC8518
Article number:SC8518
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Stock status:Special Order Item
649,00 / pcs
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