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Cell based assay, Metabolism Assay
Nicotinamide adenine dinucleotide (NAD), which acts as a soluble electron carrier between proteins, is an important enzymatic cofactor involved in many redox reactions. NAD functions in modulating cellular redox status by controlling signaling and transcriptional events, making it and related enzymes drug targets for various metabolic disorders. This colorimetric assay is based on lactate dehydrogenase catalyzed reduction of NAD, in which the formed NADH can reduce tetrazolium salt INT to an INT-formazan product. The intensity of the INT-formazan product, which exhibits maximum absorbance at 490 nm, is proportional to the amount of NADt (NAD and NADH) or NADH in the sample. This assay is specific for NAD and NADH, and it does not detect NADP or NADPH.
Catalog number: SC8368
Stock status:Special Order Item