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Cell based assay
Phosphorylation and dephosphorylation, which are the addition and removal of phosphate (PO43-) group to and from protein molecules respectively, are important regulatory mechanisms involved in cell-cycle regulation and signal transduction. ScienCell™Malachite Green Phosphate Assay provides a simple colorimetric method for the determination of inorganic, soluble phosphate concentrations based on the complexation of malachite green oxalate with phoshomolybdate under acidic conditions. Applications of the kit include measurement of phosphate release, quantification of protein-or lipid-phosphorylation and etc. For quantification of protein or lipid bound phosphate, the phosphorylated proteins or lipids need to be hydrolyzed and neutralized before phosphate measurement are performed.
Catalog number: SC8118
Article number:SC8118
Unit:
Stock status:Special Order Item
Quality Control: Data from ScienCell™ Malachite Green Phosphate Assayof phosphate solutions with concentrationsranging form 1.5625 to 37.5 μM shows a linear relationship between OD630 nm and phosphate concentration.