The L-lactate assay relies on the LDH-catalyzed oxidation of NAD to NADH. In a coupled reaction, the generated NADH reduces a probe, producing a highly fluorescent product. The fluorescence intensity, measured at an excitation wavelength of 560 nm and an emission wavelength of 590 nm, is directly proportional to the lactate concentration in the sample. The assay demonstrates a linear response up to 50 µM L-lactate, with a detection limit as low as 1 µM.
Material Supplied by User: 10 kDa MW spin filter (Millipore Sigma Cat. no. UFC501008) or 0.5 M metaphosphoric acid (Sigma-Aldrich, Cat. no. 239275)
Quality Control: L-Lactate Assay is applied to serially diluted L-Lactate Standards with concentrations ranging from 1 to 50 μM and is used to generate a standard curve (Figure 1).
Product Use: L-Lactate Assay- Fluorescence (LAC-FL) kits can be used to measure the lactate level of samples from cells, serum, plasma, cell culture media, and tissue extracts. LAC-FL is for research use only and is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures.
Shipping: All components are shipped on dry ice.