L-Lactate Assay
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L-Lactate Assay

Cell based assay, Metabolism Assay


Catalog number: SC8308

Unit size: 100 Tests in 96-well plate

Lactate is an important intermediary in glucose metabolism. Under hypoxic or anaerobic conditions, lactate dehydrogenase (LDH) converts pyruvate, the final product of glycolysis, to lactate. Monitoring lactate levels is an adequate indicator of the balance between tissue oxygen demand and utilization, and is useful when studying cellular physiology. This colorimetric assay is based on the reduction of the tetrazolium salt, INT, in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm. The intensity of the optical density is proportional to the lactate concentration.
Article number:SC8308
Unit:pcs
 
 
Stock status:Special Order Item
483,00 / pcs
Qty:
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8308.pdf