This colorimetric assay is based on a coupled enzymatic reaction of glycerol, in which the formed hydrogen peroxide is catalyzed by peroxidase and reacts with 4-aminoantipyrine to form the product dye. The color intensity of the reaction product at 540nm is directly proportional to glycerol levelin the sample. The Glycerol Assay kit measures the glycerol level of different types of samples, such as cell lysate, tissue extracts, serum and plasma.
Product Use: This product is for research purposes only and not for use in animals, humans, or diagnostic procedures.
Quality Control: Data from Glycerol Assay kit measuring glycerol solutions with concentrations ranging from 0.0625 to 2 mM show a linear relationship between OD540 nm and glycerol concentration (Figure 1). Linear detection range is 10 μM to 2 mM in 96-well plate assay.
Shipping: Dry ice.