Glucose-6-phosphate Assay
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Glucose-6-phosphate Assay

Cell based assay, Metabolism Assay

Glucose-6-phosphate (G6P) is the first substrate in glycolysis, the hexose monophosphate shunt and both the glycogen and starch biosynthesis pathways. G6P is utilized by glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. This colorimetric assay is based on glucose-6-phosphate dehydrogenase catalyzed oxidation of G6P, in which the formed NADPH can convert a nearly colorless probe to an intensely colored product, which exhibits maximum absorbance at 440 nm, is proportional to the amount of G6P in the sample.

Catalog number: SC8398
Article number:SC8398
Stock status:Special Order Item
644,00 / pcs
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