Ammonia Assay
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Ammonia Assay

Metabolism Assay

Ammonia is an important source of nitrogen for living systems. It is produced from dietary and amino acid metabolism and is toxic to the central nervous system (CNS) at high concentrations. Ammonia is taken up by the liver and converted to urea through the urea cycle. Blood ammonia measurement has been used in the diagnosis of comas associated with hepatic dysfunction caused by cirrhosis and neoplasms.This colorimetric assay is based on the oxidization of NADPH to NADP in the presence of α-ketoglutaric acid and glutamate dehydrogenase. The ammonia level is determined by assaying the rate of NADPH oxidation, which is proportional to the reduction in absorbance at 340 nm over time.

Catalog number: SC8528
Article number:SC8528
Unit:pcs
 
 
Stock status:Special Order Item
563,00 / pcs
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8528.pdf