The interconversion between malate and oxaloacetate also constitutes the essential steps of the malate-aspartate shuttle. This colorimetric assay is based on malate dehydrogenase-catalyzed oxidation of malate, where the resulting NADH can then convert a nearly colorless probe to a colored product; the intensity of the colored product is proportional to the amount of MDH in the sample, exhibiting maximum absorbance at 440 nm. The MDH Assay kit measures the MDH activity indifferent types of samples, including tissue and cell lysate.
Product Use: This product is for research purposes only and is not for use in animals, humans, or diagnostic procedures.
Quality Control: Diluted MDH positive control is measured with the MDH Assay kit after various reaction times (Figure 1 and 2). The linear range of detection is 0.15 to 1.0 mU/ml in a 96-well plate assay.
Shipping: Dry ice.