ScienCell's Colorimetric Trypsin Activity Assay Kit (TRYP) offers a rapid and sensitive way to determine the trypsin activity in mammalian cell/tissue lysates, serum, plasma and other biological fluid samples. Briefly, trypsin cleaves the substrate and releases p-nitroanilide (pNA), a chromophore that can be measured at 405 nm using a spectrophotometer. Chymotrypsin, another structurally similar serine protease, does not interfere with this assay because it does not cleave the trypsin substrate to release pNA.
Product Use: TRYP is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is Dry ice. Upon receipt, component #8898a should be stored at 4°C and #8898b, 8898c, and 8898d should be stored at -80°C. Protect from light. Repeated freeze/thaw cycles should be avoided. Aliquot if necessary. If stored properly, the kit is good for up to 12 months.
Quality Control: Serially diluted Trypsin Positive Control samples (Cat #8898c) are measured using this kit.