Colorimetric GAPDH Assay
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Colorimetric GAPDH AssayColorimetric GAPDH AssayColorimetric GAPDH AssayColorimetric GAPDH Assay

Colorimetric GAPDH Assay

Cell based assay

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is atetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for energy and carbon molecules. Since it is ubiquitously expressed at relatively constant levels, GAPDH gene is widely used as an internal control for RNA and protein analysis.The ScienCell™ Colorimetric GAPDH Assay provides a non-radioactive method to measure the enzyme activity of GAPDH in cultured cells,based on the oxidization of β-NADH to β-NAD in the presence of 3-phosphoglyceric acid (3-PGA), adenosine 5’-triphosphate (ATP) and GAPDH. The GAPDH activity is determined by assaying the rate of NADH oxidation, which is proportional to the reduction inabsorbance at 340 nmover time (ΔA340nm/min)

Catalog number: SC8148
Article number:SC8148
Stock status:In stock
308,00 /
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