Citrate Synthase Assay
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Citrate Synthase Assay

Cell-based assays

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme catalyzes the reaction between acetyl coenzyme A (acetyl CoA) and oxaloacetic acid to form citric acid and CoA with a thiol group (CoA-SH). This colorimetric assay is based on the reaction between 5’, 5’-Dithiobis 2-nitrobenzoic acid (DTNB) and CoA-SH to form TNB, which exhibits maximum absorbance at 412 nm. The intensity of the absorbance is proportional to the citrate synthase activity. This enzyme is an exclusive marker of the mitochondrial matrix.

Catalog number: SC8318
Article number:SC8318
Stock status:In stock
946,00 / pcs
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