3D Renal Tubule Formation Kit
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3D Renal Tubule Formation Kit3D Renal Tubule Formation Kit3D Renal Tubule Formation Kit

3D Renal Tubule Formation Kit

Cell based assay

The human kidney is frequently exposed to drugs and toxic compounds, which can result in nephrotoxicity. A drug’s uptake and clearance from the kidney largely relies on a subset of membrane transporters such as organic anion transporters (OAT) 1/3, organic cation transporter (OCT) 2, and multidrug and toxin extrusion proteins (MATE) 1/2. In 2D monolayer culture, primary renal epithelial cells rapidly lose the expression of OAT1/3, OCT2, and MATE1/2, limiting the accurate predictability of a drug’s effect [1]. ScienCell has developed a 3D renal tubule formation kit (3D-RTF) using human primary renal cortical epithelial cells isolated from normal adult kidney tissue, which are then embedded in Collagen I extracellular matrix. We demonstrated that renal tubule structures are formed in a robust and reproducible manner in a 3D collagenembedded model using the 3D-RTF. 3D cultured renal epithelial cells had elevated expression levels of xenobiotic transporters such as OAT1/3, and MATE1/2 (see Figure 1 and 2). The 3DRTF provides a useful in vitro tool for accurately predicting a drug’s effect on kidney function. (SC3D-4110)
Article number:SC3D-4110
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