Principle of the test
The method involves isolating DNA from a sample of a conditioned eukaryotic cell medium. Subsequently, amplification and detection of a highly conserved region encoding 16S ribosomal RNA specific for mycoplasmas is performed by PCR. This method is used for the qualitative determination of mycoplasmas in a sample.
DNA extraction from cells
Each cell sample is thawed and washed. Cell pellet is then prepared for genomic DNA isolation and purification using a DNA isolation kit.