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Cell based assay
Angiogenesis, or the formation of new blood vessels, plays an important role in many physiological (e.g.wound healing) and pathological (e.g. tumor growth and inflammation disease) processes. As a complex multi-step process, angiogenesis involves degradation of basement membrane, proliferation and migration of endothelial cells into extracellular matrix (ECM), followed by alignment and reorganization of endothelial cells into three-dimensional tubular structure and formation of new basement membrane. Specifically, the critical step of the formation of three-dimensional tubular structure by endothelial cells is the objective of many in vivo and in vitro angiogenesis assays to screen efficacy of both pro- and antiangiogenic agents. (SC8158)
Article number:SC8158
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ScienCell™ Human umbilical vein endothelial cells (HUVECs) are cultured on ECM gel from EHS tumor cells for 2-18 hours in ScienCell™ Dulbecco's Modified Eagle Medium (DMEM) with 10 % Fetal Bovine Serum(FBS). Angiogenic tube formation is characterized by staining colorimetrically.