Total RNA, Human Adult Hepatocyte (Hepatocyte total RNA)

RNA preparation: 

Total RNA is prepared from human hepatocyte cell culture  using QC controlled standard method and the RNA has been treated with RNase-free DNase I and is dissolved in RNase free water. 

Quality control: 

1. The purity of human hepatocytes for total RNA preparation is >90 %.
2. RNA quality of A260/280 ratio was in the range 1.9-2.1
3. RNA Integrity number (RIN) >8, was determined using an Agilent 2100 Bioanalyzer.
4. Concentration of RNA was determined using Nanodrop spectrophotometer.  
5. cDNA synthesis was tested by RT-PCR. 

Application: 

1. cDNA synthesis
2. RT-PCR and qPCR
3. RNA protection assay
4. Northern blot analysis
5. Purification of mRNA for library construction
6. PCR-select cDNA subtraction
7. Microarray

Product Use:

Total RNA is for in vitro laboratory research use only.

It is not approved for use in humans or animals or for application in in vitro diagnostic procedures.

Storage: 

Store the RNA immediately at -80°C upon arrival. To ensure stability, it is recommended to aliquot the RNA into single-use fractions to avoid repeated freeze/thaw cycles.

Shipping:

Dry-ice