RNA preparation:
Total RNA prepared from freshly isolated human peripheral blood CD4 positive T cells using QC controlled standard method. The RNA has been treated with RNase-free DNase I and dissolved in RNase free water.
Quality control:
- The cell purity for total RNA preparation was >90 %.
- RNA quality of A260/280 ratio was in the range 1.9-2.1
- RNA Integrity number (RIN) >8, was determined using an Agilent 2100 Bioanalyzer.
- Concentration of RNA was determined using Nanodrop spectrophotometer.
- cDNA synthesis was tested by RT-PCR.
Application:
- cDNA synthesis
- RT-PCR and qPCR
- RNA protection assay
- Northern blot analysis
- Purification of mRNA for library construction
- PCR-select cDNA subtraction
- Microarray
Product Use:
Total RNA is for in vitro laboratory research use only.
It is not approved for use in humans or animals or for application in in vitro diagnostic procedures.
Storage:
Store the RNA immediately at -80°C upon arrival. To ensure stability, it is recommended to aliquot the RNA into single-use fractions to avoid repeated freeze/thaw cycles.
Shipping:
Dry-ice or in ethanol solution at room temperature.