Four primer sets are included in the kit. Two of them (Cat #7058a and 7058b) target coronavirus SARS-CoV-2 nucleocapsid (N) gene and spike (S) gene, respectively. The human ACE2 gene primer set (Cat #7058c) targets human angiotensin-converting enzyme 2 (ACE2) gene, which serves as a cellular receptor for SARS-CoV-2. The human ACTB gene primer set (Cat #7058d) targets human β-actin (ACTB) housekeeping gene, which serves as a normalization control for cell number quantification. All primer sets are verified to possess high specificity and efficiency near 100%, with no primer dimer formation under recommended PCR conditions.
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