The
E. coli derived 39 kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 p24 gag immunodominant regions, 77-436 amino acids.The HIV-1 p24 gag is fused to beta-galactosidase (114 kDa).
Formulation: 8M urea, 20mM Tris-HCl pH 8 & 10mM b-mercaptoethanol.
Physical Appearance: Sterile filtered colorless clear solution.
Purity: Greater than 95% as determined by HPLC analysis and SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: slyn tvatlycvhq rieikdtkea ldkikeeqnk skkkaqqaaa dtghssqvsq nypivqniqg qmvhqaispr tlnawvkvve ekafspevip mfsalsegat pqdlntmlnt vgghqaamqm lketineeaa ewdrvhpvha gpiapgqmre prgsdiagtt stlqeqigwm tnnppipvge iykrwiilgl nkivrmyspt sildirqgpk epfrdyvdrf yktlraeqas qevknwmtet llvqnanpdc ktilkalgpa atleemmtac qgvggpghka rvlaeamsqv tnsatimmqr gnfrnqrkiv kcfncgkegh iarncraprk kgcwkcgkeg hqmkdcterq anflgk.
Stability: HIV-1 p24 gag although stable at +4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Transportation method: Shipped with Ice Packs.
Applications: HIV-1 p24 gag antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
References: Title: Polyfunctional CD4+ and CD8+ T Cell Responses to Tuberculosis Antigens in HIV-1–Infected Patients before and after Anti-Retroviral TreatmentPublication: The Journal of Immunology 184.11 (2010): 6537-6544.Link: http://www.jimmunol.org/content/184/11/6537.full
Specificity: Immunoreactive with all sera of HIV-1 infected individuals.
Safety data sheet: Inquire at
info@3hbiomedical.com