The
E. coli derived 20 kDa recombinant protein genotype IIIB is a non-glycosylated polypeptide chain, containing the HIV-1 nef immunodominant regions, 3-190 amino acids. The HIV-1 nef is fused to beta-galactosidase (114 kDa) at the N-terminus.
Formulation: HIV-1 nef protein solution in 8M urea, 20mM Tris-HCl pH 8.0, 10mM b-mercaptoethanol.
Physical Appearance: Sterile filtered colorless clear solution.
Purity: Greater than 95% as determined by HPLC analysis and SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: kwskssv igwptvrerm rraepaadgv gaasqdlekh gaitssntaa tnadcawleaqeeeevgfpv tpqvplrpmt ykaavdlshf lkekgglegl ihsqrrqdil dlwiyhtqgyfpdwqnytpg pgirypltfg wcyklvpvep ekleeankge ntsllhpvsl hgmddperevlewrfdsrla.
Stability: HIV-1 nef although stable at +4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Transportation method: Shipped with Ice Packs.
Applications: HIV-1 nef antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Specificity: HIV-1 nef is immunoreactive with all sera of HIV-1 infected individuals.
Safety data sheet: Inquire at
info@3hbiomedical.com