The E. coli derived 36 kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the pol protein (intergrase) and fused with a six histidines tag.
Formulation: 1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X & 50% Glycerol.
Physical Appearance: Sterile filtered colorless clear solution.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli.
Amino acid sequence: MFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLD
CTHLEGKVILVAVHVASGYIEAEVIPAETGQETAYFILKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVI
ESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPLWKG
PAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDEDHHHHHH.
Stability: HIV-1 Integrase although stable at +4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Transportation method: Shipped with Ice Packs.
Applications: HIV-1 Integrase antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Specificity: Immunoreactive with all sera of HIV-1 infected individuals.
Safety data sheet: Inquire at info@3hbiomedical.com