Recombinant Hepatitis C Virus NS5B produced in
E. coli is a single polypeptide chain containing 155 amino acids (aa 2634-2752) and having a molecular mass of 17 kDa (NCBI Accession # NP_671491).Recombinant HCV NS5B is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Formulation: The HCV NS5B protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Physical Appearance: Sterile filtered colorless solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSPMGF SYDTRCFDST VTESDIRTEE AIYQCCDLDP QARVAIKSLT ERLYVGGPLT NSRGENCGYR RCRASGVLTT SCGNTLTCYI KARAACRAAG LQDCTMLVCG DDLVVICESA GVQED.
Stability: Store at +4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Transportation method: Shipped with Ice Packs.
Safety data sheet: Inquire at
info@3hbiomedical.com