The E. coli derived 19 kDa recombinant protein contains the HCV NS4 immunodominant regions, amino acids 1658-1863. The protein is fused with b-galactosidase (114 kDa) at N-terminus, pI 5.45.
Formulation: 20mM Tris-HCl pH 8, 8M urea.
Purity: HCV NS4 a+b protein is >95% pure as determined by 10% PAGE (coomassie staining).
Amino acid sequence: 1658 TWVLVGGVLAALAAYCLSTGCVVIVGRVVLSGKPAIIPDREVLYREFDEMEE
CSQHLPYIEQGMMLAEQFKQKALGLLQTASRQAEVIAPAVQTNWQKLETFWAKHMWNFISGIQYLAGLSTLPG
NPAIASLMAFTAAVTSPLTTSQTLLFNILGGWVAAQLAAPGAATAFVGAGLAGAAIGSVGLGKVLIDILAGYGAGVAGAL 1863.
Stability: HCV NS4 a+b although stable at +4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Transportation method: Shipped with Ice Packs.
Applications: HCV NS4 a+b antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Purification Method: HCV NS4 a+b protein was purified by proprietary chromatographic technique.
Specificity: Immunoreactive with sera of HCV-infected individuals.
Safety data sheet: Inquire at info@3hbiomedical.com