The E. coli derived recombinant protein contains the NS1 Dengue Virus full length Type-2 immunodominant regions, having an Mw of 45 kDa.The dengue protein is fused to 6xHis-Tag at C-terminus and purified by proprietary chromatographic technique.
Formulation: PBS, 25mM arginine and 1M urea.
Purity: Protein is >90% pure as determined by 10% SDS-PAGE (coomassie staining).
Amino acid sequence: DSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSV
TRLENLMWKQITPELNHILSENEVKLTIMTGDIKGIMQAGKRSLQPQPTELKYSWKTWGKAKMLSTESHNQTFLIDGPETAECPNT
NRAWNSLEVEDYGFGVFTTNIWLKLREKQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKIEKASFIEVKSCHWPKSHT
LWSNGVLESEMIIPKNFAGPVSQHNYRPGYHTQTAGPWHLGKLEMDFDFCEGTTVVVTEDCGNRGPSLRTTTASGKLITEWCCRS
CTLPPLRYRGEDGCWYGMEIRPLKEKEENLVNSLVTA
Stability: Dengue NS1 although stable at +4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Transportation method: Shipped with Ice Packs.
Applications: Each laboratory should determine an optimum working titer for use in its particular application.
Safety data sheet: Inquire at info@3hbiomedical.com