The HEK293 derived Biotinylated recombinant protein contains the Coronavirus 2019 Spike Glycoprotein S1 Receptor Binding Domain [ RBD ], Wuhan-Hu-1 strain, amino acids 319-541 fused to His-Tag & AVI tag at C-terminal and having a molecular mass of 28.7 kDa.
Formulation: CoV-2 S1 RBD protein is lyophilized from 1x PBS pH 7.4 + 10% trehalose.
Physical Appearance: Lyophilized freezed dried powder.
Purity: Protein is >90% pure as determined SDS-PAGE.
Source: HEK293 Cells.
Stability: Lyophilized Cov-2 S1 Glycoprotein RBD although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CoV2 Spike protein should be stored at +4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Transportation method: Shipped at room temperature.
Uniprot ACC number: QHD43416.1
Solubility: It is recommended to reconstitute the lyophilized CoV-2 S1 protein in sterile 18M-cm H2O at a concentration of 0.2mg/ml and not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Purification Method: Purified by Metal-Afinity chromatographic technique.
Safety data sheet: Inquire at
info@3hbiomedical.com