The HEK293 derived recombinant protein contains the Coronavirus 2019 Spike Glycoprotein S1 Receptor Binding Domain [ RBD ], Wuhan-Hu-1 strain, amino acids 319-537, having a Mw of 26.5 kDa. Cov-2 RBD is fused to His-Tag at C-terminal
Formulation: Lyophilized from a 0.2μm filtered concentrated solution in PBS pH 7.4 and 10% treaholse.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Protein is >90% pure as determined SDS-PAGE.
Source: HEK293 Cells.
Stability: Lyophilized Cov-2 RBD protein although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CoV2 RBD protein should be stored at +4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Transportation method: Shipped at room temperature.
Uniprot ACC number: QHD43416.1
Solubility: It is recommended to reconstitute the lyophilized CoV-2 S1 protein in sterile 18M-cm H2O at a conc. of 0.5mg/ml and not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions
Purification Method: Purified by Metal-Afinity chromatographic technique.
Safety data sheet: Inquire at
info@3hbiomedical.com