The E. colie derived recombinant protein contains the Coronavirus 2019 CoV-2 Nucleocapsid Phosphoprotein, Wuhan-Hu-1 strain, amino acids (1-419) having a Mw of 46.4 kDa and fused to 6xHis-Tag at C-terminal.
Formulation: CoV-2 Nucleocapsid phosphoprotein was lyophilized from 20mM Na-carbonate buffer pH 9.2.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Protein is >95% pure as determined SDS-PAGE.
Source:E. coli
Stability: Cov-2 Nucleocapsid phosphoprotein is shipped lyophilized at ambient temp. Although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution COV2 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Transportation method: Shipped at room temperature.
Uniprot ACC number: YP_009724397
Solubility: It is recommended to reconstitute the lyophilized CoV-2 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Purification Method: Purified by Metal-Afinity chromatographic technique.
Antigen Amino Acid Sequence: MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASW
FTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIW
VATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAG
NGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQE
LIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKD
KKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQAHHHH.
Safety data sheet: Inquire at info@3hbiomedical.com