Recombinant Chimeric Chagas Multiantigen (MACH) (Trypanosoma cruzi) produced in
E. coli, is a polypeptide chain of 87 a.a. with epitopes PEP-2, TcD, TcE and SAPA. The protein is fused to a 6-His-Tag and having an Mw of 9.9 kDa.
Formulation: Chimeric Chagas Multiantigen was lyophilized from 20mM Tris pH 8.5, 100mM Sodium chloride, 20% trehalose and 0.1% Sodium azide as preservative.
Purity: Protein is >90% pure as determined by metal affinity chromatography.
Stability: Store lyophilized Chagas Multiantigen at +2-+8°C. After reconstitution, store at -20°C. Prevent freeze/thaw cycles.
Transportation method: Shipped at room temperature.
Applications: Suitable for use in ELISA and Lateral flow. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Solubility: It is recommended to reconstitute the lyophilized Chimeric Chagas Multiantigen in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Purification Method: Purified by proprietary chromatographic technique.
Safety data sheet: Inquire at
info@3hbiomedical.com