Recombinant Chagas 1F8 protein (Epitope of the flagellar calcium-binding protein FCABP of Trypanosoma cruzi) produced in
E. coli, is a polypeptide chain having a total of 234 a.a (includes His-Tag). The protein is fused to a 6-His-Tag having a total Mw of 25.8 kDa and a theoretical pI of 5.39.
Formulation: 1F8 Chagas antigen was lyophilized from 20mM Hepes, 300mM NaCl, pH 8, and varying 284mM imidazole.
Purity: Protein is >90% pure as determined by SDS PAGE.
Source:Escherichia coli.Stability: Store lyophilized 1F8 Chagas antigen at +2-+8°C. After reconstitution, store at -20°C. Prevent freeze/thaw cycles.
Transportation method: Shipped at room temperature.
Uniprot ACC number: P07749
Applications: Reacts with sera from infected patients, Useful for immunoassays.
Solubility: It is recommended to reconstitute the lyophilized 1F8 Chagas protein in sterile distilled pyrogen free water at 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at
info@3hbiomedical.com