Rat IL3 is a pleiotrophic cytokine produced by activated T cells. IL3 functions via specific cell surface receptors to activate hematopoiesis by controlling the production, differentiation, and proliferation of granulocytes and monocytes. IL3 also affects the activity of mature mast cells, eosinophils, basophils, and macrophages. The high affinity IL3 receptor is composed of an alpha and beta subunit, where the IL3R alpha is a member of the cytokine receptor super family and binds to IL3 with low affinity. The beta subunit does not bind any cytokine but binds to the alpha subunit of the IL3, IL5 and GM-CSF receptors with high affinity. The receptors for IL3 can be found in bone marrow, macrophages, mast cells, megakaryocytes, and other similar cells.
Aliases- Hematopoietic growth factor, mast cell growth factor, multipotential colony-stimulating factor, P-cell-stimulating factor
Formulation- Lyophilized from a 0.2 µm filtered solution in PBS
Endotoxin Level- <1.0 EU/µg of recombinant protein as determined by the LAL method.
Storage Condition- The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at +2° to +8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.