M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B cells and T cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto-oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand-binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase. Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells. M-CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.
Product Name- Recombinant Human M-CSF (CSF1)
Aliases- Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim
- Recombinant Human Macrophage Colony Stimulating Factor (CSF1)
Formulation- Lyophilized from a 0.2 µm filtered solution in Tris and NaCl (pH 7.5)
Endotoxin Level- <1.0 EU/µg of recombinant protein as determined by the LAL method.
Storage Condition- The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at +2° to +8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.