Monocytes are the main source of secreted IL1; expressing mostly IL1-beta, while human keratinocytes express large amounts of IL1-alpha. Overall, IL1-beta is the predominant form in humans while IL1-alpha is the one in mice. Both isoforms of IL1 bind to the same receptor and therefore show similar, if not identical, biological activities. The IL1-beta precursor though must be processed before it can bind to the receptor. IL1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. A monomer, IL1B stimulates thymocyte proliferation, B-cell maturation and proliferation, and fibroblast growth factor activity.
Unit Size- 10 x 100 µg (Z100397 x 10)
Aliases- Catabolin, Lymphocyte-activating factor, LAF, Endogenous Pyrogen, EP, IL1F2
- Recombinant Human Interleukin-1 Beta (IL1B)
Formulation- Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and 0.2 µm DTT (pH 8.0)
Endotoxin Level- <1.0 EU/µg of recombinant protein as determined by the LAL method.
Storage Condition- The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at +2° to +8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.