GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the amino terminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF.
Product Name- Recombinant Human µm-CSF (CSF2)
Unit Size- 10 x 100 µg (Z100277 x 10)
Aliases- GMCSF, Granulocyte-macrophage colony-stimulating factor, CSF-2, Molgramostin, Sargramostim, pluripoietin-alpha, Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (CSF2)
Formulation- Human µm-CSF was lyophilized from a 0.2 µm filtered solution in PBS, pH 7.0.
Endotoxin Level- <1.0 EU/µg of recombinant protein as determined by the LAL method.
Storage Condition- The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at +2° to +8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.