GDNF is a disulfide-bonded homodimeric glycosylated protein of 134 amino acids. The amino acid sequences inferred from rat and human cDNAs are 93% identical. The proteins contain seven conserved cysteine residues in the same relative spacing found in all members of the TGF-beta superfamily of proteins. In embryonic midbrain cultures GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Unit Size- 10 x 100 ?g (Z101058 x 10)
Aliases- GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF
- Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)
Formulation- Recombinant Glial-Derived Neurotrophic Factor was lyophilized from a 0.2 µm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 µm Glutamic acid, pH 4.5.
Endotoxin Level- <1.0 EU/µg of recombinant protein as determined by the LAL method.
Storage Condition- The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at +2° to +8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.