aFGF and bFGF display 55% homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL-1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.
Aliases- aFGF, FGF-1, Heparin-binding growth factor 1, HBGF-1, Endothelial cell growth factor, ECGF-beta, FGFA, FGF1
Formulation- Recombinant acidic FGF was lyophilized from a 0.2 µm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 µm Glutamic acid, pH 4.5.
Endotoxin Level- <1.0 EU/µg of recombinant protein as determined by the LAL method.
Storage Condition- The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at +2° to +8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.