EMAP-II is activated during apoptosis by cleavage of pro-EMAP through caspase 8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP-II also has anti-angiogenic properties, which are explained by its pro-apoptotic effect on endothelial cells.
Aliases- EMAP II, µmAP-2, µminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, µmall inducible cytokine subfamily E member 1, SCYE1
- Recombinant Human Endothelial Monocyte-Activating Polypeptide 2 (AIMP1)
Formulation- Lyophilized from a 0.2 µm filtered 20 µm PBS,130 µm NaCl solution pH 7.5
Endotoxin Level- <1.0 EU/µg of recombinant protein as determined by the LAL method.
Storage Condition- The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at +2° to +8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.