CXCL17, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 11.5 kDa. The CXCL17 is purified by proprietary chromatographic techniques.
Synonyms: Protein Cxcl17, Cxcl17, RGD1304717, C-X-C motif chemokine 17, VEGF co-regulated chemokine 1, Vcc1, VCC-1.
Formulation: CXCL17 protein was lyophilized from a 0.2 µm filtered concentrated solution in 20mM PB, 300mM NaCl, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized CXCL17 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized CXCL17 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL17 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Measured by its ability to induce VEGF expression in mouse endothelial cells. The ED50 for this effect is typically 1-5 µg/ml.
Source:Escherichia coli. Amino acid sequence: SPNQEVARHH GDQHQAPRRW LWEGGQECDC KDWSLRVSKR KTTAVLEPPR KQCPCDHVKG SEKKNRRQKH HRKSQRPSRT CQQFLKRCQL ASFTLPL.
Uniprot ACC#: D4A875
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com