Vascular Endothelial Growth Factor, Rat Recombinant, produced in yeast is a disulfide-linked homodimer consisting of 2x165 amino acid polypeptide chains, having a molecular mass of approximately 25.7 kDa each.VEGF is purified by proprietary chromatographic techniques.
Synonyms: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Formulation: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 90% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vascular Endothelial Growth Factor should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 was measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells and was found to be 0. 75‑3. 75 ng/ml.
Source: Saccharomyces cerevisiae
Amino acid sequence: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.
Uniprot ACC#: Q00731
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com