The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a. A C-terminal fusion of Flag-Tag (1 aa N-terminal+8 aa C-terminal). The TFF3 Rat produced in
Escherichia coli, is 7.7 kDa protein containing a total of 68 amino acid residues.
Synonyms: Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1. B, rP1. B, Tff3, Itf.
Formulation: TFF3 protein filtered (0. 4µm) and lyophilized in 0.5mg/ml in 20mM TRIS and 50mM NaCl, pH 7. 5.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Solubility: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at +4°C for a limited period of time; it does not show any change after two weeks at +4°C.
Source:Escherichia coli. Amino acid sequence: MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK.
Uniprot ACC#: Q03191
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com