Sonic HedgeHog Recombinant Rat produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.9 kDa. SHH is purified by proprietary chromatographic techniques.
Synonyms: SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.
Formulation: SHH is lyophilized from a sterile (0.2 µm) filtered solution containing 10 mM sodium phosphate, pH 7. 5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized SHH in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Human Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Source:Escherichia coli. Amino acid sequence: MIIGPGRGFG KRQHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKARI HCSVKAENSV AAKSDG.
Uniprot ACC#: Q15465
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com