OSM, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 214 amino acids and having a molecular mass of 24.3 kDa. The OSM is purified by proprietary chromatographic techniques.
Synonyms: Oncostatin-M, OSM, OncoM.
Formulation: OSM protein was lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Oncostatin M in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Oncostatin M although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Oncostatin should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of rat C6 cells is < 5 µg/ml, corresponding to a specific activity of > 200 units/mg.
Source:Escherichia coli. Amino acid sequence: KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SR RHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR.
Uniprot ACC#: Q65Z15
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com