MCP-3, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.5 kDa. The MCP-3 is purified by proprietary chromatographic techniques.
Synonyms: C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, MCP-3, Small-inducible cytokine A7, Ccl7, Mcp3, Scya7.
Formulation: MCP-3 protein was lyophilized from a 0.2 µm filtered concentrated solution in 20mM PB, 300mM NaCl, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized MCP-3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized MCP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCP3 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50.0-300 ng/ml.
Source:Escherichia coli. Amino acid sequence: QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP.
Uniprot ACC#: Q9QXY8
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com