Midkine, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated, polypeptide chain containing 120 amino acids and having a molecular mass of 13.2 kDa. The Midkine Rat is purified by proprietary chromatographic techniques.
Synonyms: Midkine, MK, Mdk.
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Midkine Rat in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Midkine Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Midkine Rat should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
Source:Escherichia coli. Amino acid sequence: VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD.
Uniprot ACC#: Q9R1S9
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com