MCSF, Rat Recombinant, produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254). M-CSF is disulfide-linked homodimer containing 2 x 222 a. A chains.
Synonyms: CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
Formulation: The recombinant MCSF was lyophilized after extensive dialysis against PBS.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Solubility: It is recommended to reconstitute the MCSF in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.
Source: HEK293
Uniprot ACC#: Q8JZQ0
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com