KGF 2, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 20.0 kDa. The KGF 2 is purified by proprietary chromatographic techniques.
Synonyms: FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10.
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in 1×PBS, pH 7.4 containing 5% trehalose.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized KGF 2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized KGF 2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KGF 2 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5ng/ml.
Source:Escherichia coli. Amino acid sequence: QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
Uniprot ACC#: P70492
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com